Medium data
Search for medium no. = [1029].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1029 RUMEN BACTERIA MEDIUM
| Mineral solution (see below) | 38.0 | ml |
| K2HPO4 | 0.3 | g |
| Trypticase peptone (BD-BBL) | 2.0 | g |
| Yeast extract | 0.5 | g |
| VFA solution (see Medium No. 133) | 3.1 | ml |
| Hemin | 1.0 | mg |
| Glucose | 0.5 | g |
| Maltose | 0.5 | g |
| Cellobiose | 0.5 | g |
| Starch, soluble | 0.5 | g |
| Glycerol | 0.5 | g |
| Na2CO3 | 4.0 | g |
| Resazurin | 1.0 | mg |
| L-Cysteine·HCl·H2O | 0.25 | g |
| Na2S·9H2O | 0.25 | g |
| Distilled water | 960.0 | ml |
Adjust pH to 6.7 - 6.8; gas atmosphere: 100% CO2.
Dissolve ingredients except carbohydrates, Na2CO3, cysteine and sodium sulfide in distilled water, boil for 5 min, then cool to room temperature while gassing with 100% CO2. Add Na2CO3 and equilibrate the medium with the gas to pH 6.8. Distribute under CO2 in rubber-stoppered tubes (i.e. Hungate tubes) and autoclave. Thereafter add carbohydrate mixture, cysteine and sodium sulfide from concentrated sterile stock solutions (prepared anaerobically under N2 gas and autoclaved).
Mineral solution:
| KH2PO4 | 6.0 | g |
| NaCl | 12.0 | g |
| (NH4)2SO4 | 6.0 | g |
| CaCl2·2H2O | 1.6 | g |
| MgSO4·7H2O | 2.5 | g |
| Distilled water | 1.0 | L |
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