Medium data

Search for medium no. = [1227].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1227 ZESTOSPHAERA MEDIUM

Modified Brock's salt base solution (see Medium No. 165)	`1.0`	L
Na₂S₂O₃·5H₂O	`2.0`	g
NaOH	`0.16`	g
Resazurin	`1.0`	mg
Trace mineral solution (see Medium No. 446)	`10.0`	ml
Distilled water	`1.0`	L

Heat the medium to boiling while bubbling with a N₂-CO₂ (4:1, v/v) gas mixture. After boiling, cool the medium in a water bath. Continue bubbling with the same gas mixture. While the medium is still warm, add the following:

Yeast extract (Oxoid)	`1.0`	g
Tryptone	`1.0`	g
Casamino acids (BD-Difco)	`0.5`	g
L-Cystein·HCl·H₂O	`0.0625`	g

After the medium is cooled to room temperature, adjust pH to 6.0 - 6.1. Dispense the medium into culture vessels (e.g., 10 ml in Balch type tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave.