Medium data

Search for medium no. = [1319].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1319 MODIFIED ACETOBACTER MEDIUM

NH₄Cl	`1.0`	g
KH₂PO₄	`0.33`	g
K₂HPO₄	`0.45`	g
NaCl	`10.0`	g
MgSO₄·7H₂O	`0.1`	g
Trace minerals (see Medium No. 151)	`10.0`	ml
Yeast extract	`2.0`	g
Resazurin	`0.5`	mg
Distilled water	`910.0`	L

Mix components thoroughly and autoclave under a N₂-CO₂ (80:20, v/v) gas atmosphere. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized and):

8% NaHCO₃ solution*	`50.0`	ml
1.0 M Fructose solution	`20.0`	ml
Trace vitamins* (see Medium No. 197)	`10.0`	ml

Distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under the same gas mixture and seal with butyl rubber stoppers. Add per liter the following solutions (autoclaved and stored under N₂):

5% Na₂S·9H₂O solution	`10.0`	ml
5% L-Cysteine·HCl·H₂O solution	`10.0`	ml