Medium data
Search for medium no. = [412].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
412 Fe(III)-REDUCER FRESHWATER ENRICHMENT MEDIUM
Poorly crystalline iron(III) oxide (see Medium No. 411) | 100.0 | mM |
MgCl2·6H2O | 0.33 | g |
CaCl2·2H2O | 0.33 | g |
KCl | 0.33 | g |
KH2PO4 | 0.33 | g |
NH4Cl | 0.25 | g |
NaHCO3 | 2.5 | g |
Na2SeO4 | 18.9 | mg |
Trace vitamins (see Medium No. 197) | 10.0 | ml |
Trace mineral solution (see Medium No. 411) | 10.0 | ml |
L-Cysteine·HCl·H2O | 0.044 | g |
FeCl2·4H2O | 0.26 | g |
Add components except L-cysteine·HCl·H2O and FeCl2·4H2O to distilled water, bring volume to 1.0 L and adjust pH to 7.0. Distribute the medium into appropriate culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a H2-CO2 (4:1, v/v) gas mixture, sparge with the same gas mixture for 10 - 15 min, seal with butyl rubber stoppers and autoclave. Separately autoclave concentrated (100- or 200-fold) L-cysteine·HCl·H2O and FeCl2·4H2O solutions under a N2 gas atmosphere. Prior to inoculation, add the sterile L-cysteine·HCl·H2O and FeCl2·4H2O solutions. Pressurize the inoculated vessels to 100 kPa H2-CO2 (4:1, v/v) and cultivate without shaking.
Comment: For strain JCM 12379, inoculate with a 10% inoculum.
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