Medium data

Search for grmd=[439].
439   DVO5 MEDIUM

NaCl  1.0     g
MgCl2·6H2O  0.4     g
CaCl2·2H2O  0.1     g
NH4Cl  0.25    g
KH2PO4  2.0     g
KCl  0.5     g
Yeast extract  0.5     g
Na2SO4  4.0     g
Trace element solution (see below)  1.0     ml
Vitamin solution (see Medium No. 403)  1.0     ml
Thiamine solution (see Medium No. 403)  1.0     ml
Vitamin B12 solution (see Medium No. 403)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
Sodium lactate  2.24    g
Resazurin  1.0     mg
Sodium dithionite  0.1     g
Dissolve components, except Vitamin solution, Thiamine solution, Vitamin B12 solution, sodium lactate and sodium dithionite, in 900 ml distilled water and adjust pH to 7.0. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 9 ml of the medium in Hungate tubes) under N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Separately autoclave 0.2 M sodium lactate under a N2 atmosphere. After cooling, add 1/9 volume of the sodium lactate solution, and filter-sterilized Vitamin solution, Thiamine solution and Vitamin B12 solution to the medium. Readjust pH to 7.0, if necessary. Prior to use, reduce the medium with a freshly prepared, filter-sterilize 1% sodium dithionite solution.
Trace element solution:
HCl (25%)  12.5     ml
FeSO4·7H2O  2.1     g
H3BO3  30.0     mg
MnCl2·4H2O  100.0     mg
CoCl2·6H2O  190.0     mg
NiCl2·6H2O  24.0     mg
CuCl2·2H2O  2.0     mg
ZnSO4·7H2O  144.0     mg
Na2MoO4·2H2O  36.0     mg
Distilled water  987.0     ml