Medium data

Search for grmd=[748].

748 METALLOSPHAERA CUPRINA MEDIUM

(NH₄)₂SO₄	`3.0`	g
KH₂PO₄	`0.5`	g
MgSO₄·7H₂O	`0.5`	g
KCl	`0.1`	g
Ca(NO₃)₂	`0.01`	g
Yeast extract	`2.0`	g
Tryptone (Difco)	`0.5`	g
Distilled water	`1.0`	L

Adjust pH to 3.5 and autoclave. After cooling, aseptically add 2.0 ml of Trace element solution (see below).
Trace element solution (filter-sterilized):

FeCl₃·6H₂O	`1.1`	g
CuSO₄·5H₂O	`0.05`	g
H₃BO₃	`0.2`	g
MnSO₄·H₂O	`0.2`	g
Na₂MoO₄·2H₂O	`0.08`	g
CoCl₂·6H₂O	`0.06`	g
ZnSO₄·7H₂O	`0.09`	g
Distilled water	`1.0`	L