Medium data

Search for medium no. = [896].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

896   MODIFIED BSK MEDIUM

HEPES  3.0     g
Trisodium citrate·2H2O  0.35    g
Glucose  2.5     g
Sodium pyruvate  0.4     g
N-Acetyl-D-glucosamine  0.2     g
NaHCO3  1.1     g
MEM alpha Modification (BioWest)  5.2     g
TC Yeastolate (BD-Difco)  1.0     g
Probumin Universal Grade (Millipore)  25.0     g
Neopeptone (BD-Difco)  2.5     g
Rabbit serum sterile non-hemolyzed young (Pel-Freez code No. 31125)  40.0     ml
Adjust pH to 7.2 with NaOH, fill up to 584 ml with distilled water and filter-sterilize with 0.22 µm PES filter.



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