Medium data

Search for grmd=[721].
721   THIOALBUS DENITRIFICANS MEDIUM

NaCl  20.0     g
MgCl2·6H2O  0.4     g
CaCl2·2H2O  0.1     g
NH4Cl  0.2     g
KH2PO4  1.0     g
KCl  0.5     g
Yeast extract  0.1     g
Distilled water  1.0     L
Mix components and adjust pH to 7.5. Dispense the medium into culture vessels, purge with N2 for 5 min, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions (filter-sterilized and stored anaerobically):
Vitamin solution (see Medium No. 403)  1.0     ml
Trace element solution (see Medium No. 439)  1.0     ml
8% NaHCO3 solution  5.0     ml
1 M NaNO3 solution  5.0     ml
1 M Na2S2O3 solution  5.0     ml